Nasal Spray Dilution Calculator
Calculate peptide concentration and dose per spray for nasal spray formulations.
Standard Base Ingredients
- Purified/sterile water — the solvent
- Sodium chloride (NaCl) at 0.9% w/v — makes it isotonic with nasal mucosa
- Preservative — bacteriostatic water (0.9% benzyl alcohol) OR benzalkonium chloride at 0.01%
Key Facts
- Standard metered-dose nasal spray pumps deliver 0.1 mL per spray (actuation)
- Bacteriostatic water keeps solution stable ~30 days refrigerated
- Sterile water (no preservative) — use within 7 days refrigerated
- Never freeze reconstituted solution (ice crystals destroy peptide structure)
Reconstitution Technique
- Let lyophilized vial reach room temperature (15–20 min)
- Add diluent slowly down the inside wall of the vial
- Gently swirl, never shake (shear forces cause peptide aggregation)
Your Formulation
1.0 mg/mL (0.1%) — fixed
Diluent Volume to Add
To deliver 300 mcg in 1 spray
3.33 mL
To deliver 300 mcg in 2 sprays
6.67 mL
Based on a fixed 0.1 mL metered spray. Diluent volume is calculated so the target dose is delivered in the specified number of sprays.
Common Dilutions Reference
Standard formulations for reference. All calculations based on 0.1 mL per spray.
| Peptide Amount | Diluent Volume | Concentration | Dose per Spray |
|---|---|---|---|
| 5 mg | 5 mL | 1 mg/mL (0.1%) | 100 mcg |
| 10 mg | 10 mL | 1 mg/mL (0.1%) | 100 mcg |
| 10 mg | 5 mL | 2 mg/mL (0.2%) | 200 mcg |
| 30 mg | 10 mL | 3 mg/mL (0.3%) | 300 mcg |
| 30 mg | 15 mL | 2 mg/mL (0.2%) | 200 mcg |
| 30 mg | 30 mL | 1 mg/mL (0.1%) | 100 mcg |
This calculator is for research reference purposes only. Always verify formulations independently. Not intended as medical advice.